FMI Air, a scheduled airline, has launched three daily flights between Yangon and Nay Pyi Taw.
The firm, which has 170 staff in Myanmar, also launched another flight to Mandalay.
It uses a Bombardier CRJ200 and expects to expand its service and fleet.
"The Bombardier is small but fast as it's a jet. It carries 50 passengers. The country has never used the aircraft before. The pilots, engineers and technicians are all from overseas. Four or five months later, we will hire local people and train them," said FMI Air's CEO Bruce Nobles.